Primary structure of porcine spleen ribonuclease: sequence homology.
نویسندگان
چکیده
منابع مشابه
The primary structure of porcine pancreatic ribonuclease. I. The distribution and sites of carbohydrate attachment.
Reduced, S-aminoethylated porcine ribonuclease was subjected to tryptic hydrolysis. A sequence of fractionation steps that included gel filtration and chromatography over SE-Sephadex C-50, Dowex 50-X2, and Dowex l-X2 afforded a series of glycopeptide fractions which were recognized to derive from three separate regions of the molecule. Heterogeneity of the attached polysaccharide chains preclud...
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Porcine spleen DNase II, a lysosomal acid hydrolase, is a noncovalently linked azb heterodimer (Liao, T.-H. (1985) J. Biol. Chem. 260, 10708–10713). The a subunit, after disulfide cleavage, yields two chains, a1 and a2. The complete amino acid sequences of the a1, b, and a2 chains were elucidated by protein sequencing, and the pairings of one interchain disulfide between a1 and a2 and of three ...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 1997
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.1997_4371